Isolation and indentification of phosphate solubilizing fungi. Isolation of phosphate solubilizing bacterial strain. However, though results of this study make these fungi. The clear halo zone around the colonies was considered as positive result for psolubilization. A total of twenty two rhizospheric fungi were isolated from different healthy crop plants of hassan, karnataka, india and tested for its efficacy to solubilize. Pdf isolation of phosphate solubilizing fungi from. The two psf, talajx04 and aspnjx16, were identified as talaromyces aurantiacus and aspergillus neoniger respectively, based on morphologic and phylogenetic characteristics. Phosphate solubilizing fungi isolated and characterized from teff.
A study was conducted to isolate phosphate solubilizing bacteria psb from aerobic rice grown in penang malaysia and to determine some biochemical properties of the isolates such as, organic acids, enzymes, indoleacetic acid iaa, siderophore production and its antagonistic effect against pathogen rhizoctonia solani. Phosphate solubilization all of the isolated bacterial isolates were checked for phosphate solubilization on pikoviskaya agar plates 1618. Additionally sayer and gadd 1997, certain fungi are able to immobilize and release p by precipitating insoluble metal. Isolation screening and selection of phosphate solubilizing. Impact on growth and development of economically important plants. Inorganic phosphate solubilization is one of the major mechanisms of plant growth promotion by plant associated bacteria. In this study, we first isolated two phosphate solubilizing fungi psf from moso bamboo rhizosphere soil in acid soil of jiangxi province, china. Isolation of phosphate solubilizing fungi from rhizosphere soil and its effect on seed growth parameters of different crop plants article pdf available november 2016 with 583 reads how we. Isolation and evaluation of rock phosphate solubilizing. Therefore, this study aimed to isolate zinc phosphate solubilizing fungi zpsf from rhizospheric soil cultivated with economic crops. Isolation and identification of phosphate solubilizing. Plant growth promotion induced by phosphate solubilizing. Aug 25, 2018 phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms. A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also.
Isolation and characterization of phosphate solubilizing. Isolation and identification of potential phosphate. Isolation and characterization of bacteria and fungi from. Isolation and charecterisation of seaweed endophytic fungi as. In this study, two psf, talajx04 and aspnjx16, were isolated from the rhizosphere soil of moso bamboo phyllostachys edulis widely distributed in pdeficient areas in china and identified as talaromyces aurantiacus. Those fungal colonies showing clear zone around the colonies were selected. Isolation of phosphate solubilizing fungi the isolation of phosphate solubilizing fungi from each type of oil palm empty fruit bunches was done by the pouring method on pikovskaya 1948 medium with tricalcium phosphate ca 3 po 3 2 as an insoluble organic. Isolation and evaluation of rock phosphate solubilizing fungi as potential biofertilizer s. However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. Isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. After confirming the phosphate solubilizing ability on solid medium, the phosphate solubilization were. Collected rhizosphere soil samples were used for the isolation of phosphate solubilizing fungi on pikovskayas pkv agar medium, containing the following gl. This research is to isolate and select phosphate solubilizing soil fungi from kanchanaburi area. Phosphate solubilizing bacterial strain isolated from a jute mill effluent mixing area of ganga river water at bansberia 2258.
The phosphate solubilization ability of the fungi was analyzed by determining the phosphate solubilization efficiency e of each isolate. A number of bacterial and fungal isolates from soil were screened for phosphorus solubilization. After screening, 14 fungal isolates were obtained, which were further screened based. Oct 26, 2015 a number of bacterial and fungal isolates from soil were screened for phosphorus solubilization. Abid ullah 1, hafsa mushtaq 2, usman ali 1, hakim 1, ehsan ali 3, samavia mubeen2 1department of plant science and technology, huazhong agricultural university, wuhan, hubei, p. A study was conducted to isolate phosphatesolubilizing bacteria psb from aerobic rice grown in penang malaysia and to determine some biochemical properties of the isolates such as, organic acids, enzymes, indoleacetic acid iaa, siderophore production and its antagonistic effect against pathogen rhizoctonia solani. This involves bacteria releasing organic acids into the soil which solubilize the phosphate complexes converting. Arbuscular mycorrhizal fungi amf and phosphate solubilizing bacteria psb could interact synergistically because psb solubilize sparingly available phosphorous compounds into orthophosphate.
Chapter 3 isolation, screening, morphological and biochemical. Impact of heavy metals and initial ph variations on the. The phosphate solubilizing efficiency of isolated psb strain kupsb12 in pikovskayas broth indicated that the strain efficiently solubilized inorganic phosphate in the medium containing 0. Phosphate solubilization potential of rhizosphere fungi. Anantharaman cas in marine biology, faculty of marine sciences, annamalai university, parangipettai608502, tamil nadu, india. Fungi isolation phosphate solubilizing fungi psf were isolated from 25 subsamples of the soils using selective media, pikovskaya medium pvk. Mehta department of biotechnology, research and development centre, gsfc ltd vadodara, india abstract rock phosphate is the cheapest and abundant phosphatic fertilizer available but due to its sparse solubility. The screening of phosphate solubilizing fungi on pkv media was done after incubation, the observed colonies were morphologically distinct, both with and without halos. Therefore, this study aimed to isolate zincphosphatesolubilizing fungi zpsf from rhizospheric soil cultivated with economic crops. Isolation, screening and characterization of phosphate. E solubilization diameter growth diameter x 100 by 15. Optimization for rock phosphate solubilization by phosphate. Isolation of phosphate solubilizing microorganism psms from. One hundred and forty five fungi isolates were incubated on.
Contrasting nutrient agar and calcium phosphate mediums represented the much higher mpn value on. Phosphate solubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Isolation and charecterisation of seaweed endophytic fungi as an efficient phosphate solubiizers noorjahan a, b. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan.
Pdf phosphate solubilizing fungi isolated and characterized from. Phosphatesolubilizing bacteria and fungi in various cultivated and virgin alberta soils. Pdf isolation of phosphate solubilizing fungi from rhizosphere soil. Request pdf isolation and evaluation of rock phosphate solubilizing fungi as potential biofertilizer rock phosphate is the cheapest and abundant phosphatic fertilizer available but due to its. Solubilization of inorganic phosphate by phosphate. Percentage of silicate solubilizing bacteria was checked for each soil sample and calculated as follows. Based on the result of in vitro studies, the inoculation effect of phosphate solubilizing microorganisms psms isolates on the nodulation, growth and phosphorus content of chickpea plants was studied under pot culture conditions using a efficient strain of pseudomonas sp. Among them about 78 isolates was found from the different rhizosphere of sunflower plants. Isolation and evaluation of rock phosphate solubilizing fungi as.
The present study was aimed at isolating and characterizing phosphate solubilizing fungi from different rhizospheres using both solid and liquid pikovskaya pvk medium. The analysis of phosphate solubilizing activity of the selected isolates were conducted qualitatively and quantitatively by plate screening method and broth culture method. Phosphatesolubilizing fungi psf generally enhance available phosphorus p released from soil, which contributes to plants p requirement, especially in plimiting regions. Phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants.
One fungal strain was found to be efficient phosphorus solubilizer in pikovskaya medium. Mehta department of biotechnology, research and development centre, gsfc ltd vadodara, india abstract rock phosphate is the cheapest and abundant phosphatic fertilizer available but. Phosphate solubilizing fungi isolated and characterized from teff rhizosphere soil collected from north showa zone, ethiopia. The use of plant growth promoting bacterial inoculants as live microbial biofertilizers provides a promising alternative to chemical fertilizers and pesticides. Now, it is important to identify betterfunctioning p solubilizing fungi. Number of psb among total psm in north iranian soil was around 88 % fallah, 2006. Isolation and screening of silicate bacteria from various. Qualitative screening of psm an efficient protocol was developed for qualitative screening of phosphatesolubilizing bacteria, based upon visual observation. Isolation and characterization of two phosphatesolubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and ph environments. Nagalakshmi department of microbiology, kanchi shri krishna college of arts and science, kilambi, kanchipuram631551, tn, india corresponding author abstract introduction phosphorus p is a major growthlimiting. Isolation of phosphate solubilizing bacteria and fungi from. Qualitative screening of psm an efficient protocol was developed for qualitative screening of phosphate solubilizing bacteria, based upon visual observation. Screening, isolation, biochemical and plant growth promoting characterization of endophytic bacteria. Pikovskayas agar amended with tricalcium phosphate was used to isolate the psolubilizing fungi.
Phosphate solubilizing bacteria psb have the potential to enhance phosphateinduced immobilization of metals to remediate contaminated soil. Impact of phosphatesolubilizing fungi on the yield and phosphorus. Many bacteria and fungi are able to improve plant growth by solubilizing inorganic and organic phosphates in the soil. Screening, isolation, biochemical and plant growth promoting. The two psf, talajx04 and aspnjx16, were identified as talaromyces aurantiacus and aspergillus neoniger respectively, based on. Pdf identification of phosphatesolubilizing microorganisms. Isolation of phosphorussolubilizing fungus from soil to. Some important cultural conditions were optimized, and optimal conditions for temperature, glucose. Isolation and screening of phosphate solubilizing bacteria wide number of bacterial isolates was found from the different agricultural lands of surat and bharuch district in gujarat, india. Solubilization of inorganic phosphate by rhizospheric fungi.
Pigeon pea cajanus cajan is an important food crop, a principal source of protein in the indian diet, and a very popular food in developing tropical countries. A total of 59 fungi were originally obtained and were screened at the. Soil bacteria and fungi mediate soil processes such as decomposition, nutrient mobilization and mineralization. Isolation and characterization of phosphate solubilizing microbes from agricultural soil t. Phosphatesolubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Fungi and bacteria have the ability to solubilizing these compounds 4 isolation of microbial strains. Isolation of phosphate solubilizing fungi from rhizosphere soil and its. Isolation and characterization of two phosphatesolubilizing. Colony morphology of phosphorus solubilizing fungal.
Exploration of phosphate solubilizing microorganisms has. Isolation of psolubilizing fungi was carried out by serial dilution and pour plate technique. Interaction between phosphate solubilizing bacteria and. Islam et al isolation of phosphate solubilizing fungi from subtropical soils9145applied ecology and environmental research 174. Isolation and characterization of phosphatesolubilizing. Phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms. In the present study, three phosphatesolubilizing fungi c.
Phosphate solubilizing bacteria are frequently used as plant growth promoters. Research article phosphate solubilization potential of. The selected strain was identified as aspergillus niger based on morphological and microscopic studies. Phosphatesolubilizing microorganisms associated with the. Pdf isolation and characterization of two phosphate. Isolation of phosphate solubilizing fungi phosphate solubilizing fungi psf were isolated from each sample by serial dilution and spread plate method gaur, et al. Phosphatesolubilizing bacteria are frequently used as plant growth promoters. In this study, we first isolated two phosphatesolubilizing fungi psf from moso bamboo rhizosphere soil in acid soil of jiangxi province, china.
Under the situation, many phosphate solubilizing microorganisms have been isolated from different environments and phosphorus availability to plants yu et al. Isolation of phosphate solubilizing bacteria and fungi. Isolation and indentification of phosphate solubilizing fungi from rhizosphere soil free download as pdf file. The serially dilution from 10 to 105 was achieved by trans ferring 1ml of mushroom residue solution from each preceding attenuation stage to the next. The collected soil samples were serially diluted using sterile water blanks and plated on rose bengal agar medium. Isolation of phosphate solubilizing bacteria and fungi from rhizospheres soil from banana plants and its effect on the growth of amaranthus cruentus l. Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soilbased agriculture. Because of the negative charge of phosphate ions, they are quickly absorbed after weathering of clays or detritus particles, forming insoluble forms of aluminum, calcium, or iron phosphates, all unavailable to mangroves. Phosphate solubilization potential of rhizosphere fungi isolated. Among the whole microbial population in soil, psb constitute 1 to 50 %, while phosphorus solubilizing fungi psf are only 0. Now, it is important to identify betterfunctioning psolubilizing fungi. Isolation and characterization of two phosphatesolubilizing fungi. Lecturer5 department of microbiology malanakara catholic college mariagiri, kaliakkavilai 629053.
Isolation and characterization of a phosphatesolubilizing. Isolation of phosphate solubilizing microorganism psms. Phosphorus p is one of the major bioelements limiting agricultural production. Under the situation, many phosphatesolubilizing microorganisms have been isolated from different environments and phosphorus availability to plants yu et al.
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